Journal:

Article Title: Quality Control Limits for Broth Microdilution Susceptibility Tests of Ten Antifungal Agents

doi:

Figure Lengend Snippet: Distribution of MICs reported by eight participating facilities each testing two control strains on 10 separate days

Article Snippet: For most drugs, the 48-h MICs were approximately one doubling concentration greater than those recorded after 24 h. Every effort was made to select MIC ranges that included at least 95% of the recorded values and that occasionally required a four-dilution range around a clearcut mode. table ft1 table-wrap mode="anchored" t5 caption a7 Antifungal agent and control strain a Incubation time (h) No. of times the following MICs (μg/ml) were reported ( n = 240) b : ≤0.016 0.03 0.06 0.12 0.25 0.5 1.0 2.0 4.0 8.0 16 32 64 128 Amphotericin B C. parapsilosis 24 7 [1 120 109 3] 48 20 [1 88 123 8] C. krusei 24 [2 141 97] 48 [10 202 28] Flucytosine C. parapsilosis 24 [43 c 152 43] 1 1 48 4 c [12 189 34] 1 C. krusei 24 1 5 [36 119 79] 48 1 [74 162 3] Fluconazole d C. parapsilosis 24 1 2 [4 55 91 15] 48 1 2 1 [42 119 41] C. krusei 24 [5 95 76 4] 48 [4 108 98 0] Voriconazole C. parapsilosis 24 [9 86 141 4] 48 [21 113 83 3] C. krusei 24 4 [17 76 139 4] 48 [0 110 125 5] Ketoconazole C. parapsilosis 24 6 [7 106 108 13] 48 2 [23 114 82 17] 2 C. krusei 24 1 10 [32 74 123 0] 48 [25 143 70] 1 Itraconazole C. parapsilosis 24 5 4 [34 129 66] 1 48 5 [28 149 57] 1 C. krusei 24 1 9 [23 68 136 3] 48 [23 170 46] Caspofungin (MK0991) C. parapsilosis 24 6 1 1 [29 159 44] 48 1 5 [11 143 67 2] 9 2 c C. krusei 24 3 [22 98 116 1] 48 4 [22 164 48] 2 Ravuconazole (BMS 207147) C. parapsilosis 24 [9 93 121 7] 10 48 3 [8 114 92 22] 1 C. krusei 24 6 10 [17 72 96 39] 48 [83 152 5] Posaconazole (SCH 56592) C. parapsilosis 24 7 [36 119 77] 1 48 1 1 [15 161 61] 1 C. krusei 24 [12 51 107 70] 48 1 [3 93 140 3] LY 303366 C. parapsilosis 24 4 3 2 [43 111 77 0] 48 1 [31 83 123 2] C. krusei 24 1 [8 130 75 23] 2 1 48 1 2 [59 108 44 14] 10 2 Open in a separate window a C. parapsilosis ATCC 22019 and C. krusei ATCC 6258. b Proposed QC limits are indicated by brackets. c MICs outside the range of flucytosine or caspofungin that were actually tested. d Data from one laboratory were excluded because MICs of the control drug, amphotericin B, were outside of the proposed limits; another laboratory reported only 48-h readings.

Techniques: Control, Incubation

Journal:

Article Title: Development of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples from Neutropenic Patients

doi: 10.1128/JCM.41.5.1811-1818.2003

Figure Lengend Snippet: PCR results for fungal and bacterial strains

Article Snippet: The cross-reactivities of the primers and the hybridization probes with human DNA were excluded by testing of DNA from 10 healthy control persons by the LightCycler PCR assay. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Organism (strain) LightCycler PCR result a Fungal strains Aspergillus fumigatus (DSM 819 CS) + Aspergillus flavus (DSM 1959) − Aspergillus terreus (DSM 1958) − Aspergillus niger (DSM 63263) − Aspergillus versicolor (DSM 1943) − Aspergillus clavatus (DSM 3410) + Emericella nidulans (DSM 820) − Penicillium chrysogenum (DSM 844) − Penicillium expansum (DSM 1282) − Penicillium fumiculosum (DSM 1944) − Paecilomyces variotii (DSM 1961) − Rhizopus oryzae (DSM 854) − Candida glabrata (DSM 70614) − Candida tropicalis (DSM 1346) − Candida parapsilosis (DSM 70126) − Candida albicans (DSM 1386) − Candida krusei (DSM 70079) − Fusarium proliferatum (DSM 848) − Bacterial strains Streptococcus mitis (DSM 20568) − Streptococcus pneumoniae (DSM 20566) − Staphylococcus aureus (DSM 799) − Staphylococcus epidermidis (DSM 709) − Pseudomonas aeruginosa (ATCC 27853) − Klebsiella pneumoniae (DSM 681) − Enterobacter cloacae (DSM 6234) − Serratia marcescens (DSM 1636) − Haemophilus influenzae (DSM 4690) − Proteus mirabilis (DSM 788) − Escherichia coli (DSM 787) − Enterococcus faecalis (DSM 2570) − Enterococcus faecium (DSM 2146) − Open in a separate window a Results from at least four separate experiments are shown.

Techniques: